Marino Lab – Biomolecular Mass Spectrometry and Proteomics

Fabio Marino

Assistant Professor

Our group focuses on antigen discovery and immunobiology, aiming to deepen our understanding of HLA class I and II antigen processing and presentation using mass spectrometry–based proteomics and immunopeptidomics. By studying these processes in both immune and diseased cells, we seek to improve clinical applications, identify targetable HLA-bound antigens, and uncover how T cells recognize antigens. We also develop and apply advanced analytical workflows, including automation, liquid chromatography, ion mobility, mass spectrometry, alternative fragmentation techniques, and bioinformatics. Our lab is part of the Biomolecular Mass Spectrometry and Proteomics group within the Utrecht Institute for Pharmaceutical Sciences (UIPS), Oncode Accelerator (OA), and the Princess Máxima Center (PMC).

Awarded Grants

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2025

Recognition of Action Vitality Forms is Linked to Social Communication Traits in Autism

Valentina Cuccio, F Tambuscio, Emilio Leonardi, Francesca Isabella Famà, Chiara Carrozza, Stefania Aiello, Agrippina Campisi, Roberta Minutoli, Paola Chilà, Fabio Marino, Susan C. Campisi, Roberta Bruschetta, Annalisa Pelosi, Alessandra Sciutti, Marilina Mastrogiuseppe, Liliana Ruta, Gennaro Tartarisco, Giovanni Pioggia, Giuseppe Di Cesare, The NEST Team, M. Boncoddo, I. Crimi, F. Di Bella, G. Lazzaro, S. Martines, A. Orsina, M. Rosano

Journal of Autism and Developmental Disorders

Increased EThcD Efficiency on the Hybrid Orbitrap Excedion Pro Mass Analyzer Extends the Depth in Identification and Sequence Coverage of HLA Class I Immunopeptidomes

Amy L. Kessler, Kyle L. Fort, Hanno C. Resemann, Péter Krüger, Cong Wang, Heiner Koch, Jan-Peter Hauschild, Fabio Marino, Albert J. R. Heck

Molecular & Cellular Proteomics

MR1-ligand cross-linking identifies vitamin B6 metabolites as TCR-reactive antigens

Thierry Schmidlin, Enas M. Behiry, Hannah Thomas, Garry Dolton, Fabio Marino, Samiul Hasan, Magdaléna von Essen, Rose M. Gathungu, Barbara Steigenberger, Hayden Selvadurai, Joseph Dukes, Paul E. Brennan, O. Brad Spiller, Jonathan D. Silk, Andrew K. Sewell, Nicola Ternette

Cell Reports Methods

Expanding the definition of MHC Class I peptide binding promiscuity to support vaccine discovery across cancers with CARMEN

Michał Waleron, Ashwin Adrian Kallor, Aleksander Pałkowski, Emilia Daghir‐Wojtkowiak, Piyush Borole, Mikołaj Kocikowski, Karol Połom, Fabio Marino, Beatriz Monterde, Michele Mastromattei, Davide Venditti, Mark Stares, Ashita Singh, Theodore Hupp, Christophe Battail, Alexander Laird, Cátia Pesquita, Luís Zapata, Stefan N. Symeonides, Ajitha Rajan, Fabio Massimo Zanzotto, Javier A. Alfaro

Preprint

2024

Exploring divergent kinematics in autism across social and non-social vitality forms

Giuseppe Di Cesare, Roberta Bruschetta, Augusto Vitale, Annalisa Pelosi, Emilio Leonardi, Francesca Isabella Famà, Marilina Mastrogiuseppe, Chiara Carrozza, Stefania Aiello, Agrippina Campisi, Roberta Minutoli, Paola Chilà, Simona Campisi, Fabio Marino, Giovanni Pioggia, Gennaro Tartarisco, Valentina Cuccio, Liliana Ruta

Scientific Reports

2020

Biogenesis of HLA Ligand Presentation in Immune Cells Upon Activation Reveals Changes in Peptide Length Preference

Fabio Marino, Aikaterini Semilietof, Justine Michaux, HuiSong Pak, George Coukos, Markus Müller, Michal Bassani‐Sternberg

Frontiers in Immunology

Deciphering the Mechanisms of Improved Immunogenicity of Hypochlorous Acid-Treated Antigens in Anti-Cancer Dendritic Cell-Based Vaccines

Michele Graciotti, Fabio Marino, HuiSong Pak, Petra Baumgaertner, Anne‐Christine Thierry, Johanna Chiffelle, Marta A. S. Perez, Vincent Zoete, Alexandre Harari, Michal Bassani‐Sternberg, Lana E. Kandalaft

Vaccines

2019

High-Throughput, Fast, and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry

Fabio Marino, Chloé Chong, Justine Michaux, Michal Bassani‐Sternberg

Methods in molecular biology

2018

Vaccine antigens modulate the innate response of monocytes to Al(OH)3

Sietske Kooijman, Jolanda Brummelman, Cécile A. C. M. van, Fabio Marino, Albert J. R. Heck, Elly van Riet, Bernard Metz, Gideon Kersten, Jeroen L. A. Pennings, Hugo D. Meiring

PLoS ONE

Minimal Information About an Immuno‐Peptidomics Experiment (MIAIPE)

Jennie R. Lill, Peter A. van Veelen, Stefan Tenzer, Arie Admon, Étienne Caron, Joshua E. Elias, Albert J. R. Heck, Miguel Marcilla, Fabio Marino, Markus Müller, Bjoern Peters, Anthony W. Purcell, Alessandro Sette, Theo Sturm, Nicola Ternette, Juan Antonio Vizcaíno, Michal Bassani‐Sternberg

PROTEOMICS

Novel identified aluminum hydroxide-induced pathways prove monocyte activation and pro-inflammatory preparedness

Sietske Kooijman, Jolanda Brummelman, Cécile A. C. M. van, Fabio Marino, Albert J. R. Heck, Geert P. M. Mommen, Bernard Metz, Gideon Kersten, Jeroen L. A. Pennings, Hugo D. Meiring

Journal of Proteomics

Abstract PL02-02: Immunopeptidomics: Accelerating the development of personalized cancer immunotherapy

Michal Bassani‐Sternberg, Chloé Chong, Fabio Marino, HuiSong Pak, David Gfeller, Markus Müller, George Coukos

Molecular Cancer Therapeutics

2017

High-throughput and Sensitive Immunopeptidomics Platform Reveals Profound Interferonγ-Mediated Remodeling of the Human Leukocyte Antigen (HLA) Ligandome

Chloé Chong, Fabio Marino, HuiSong Pak, Julien Racle, Roy Thomas Daniel, Markus Müller, David Gfeller, George Coukos, Michal Bassani‐Sternberg

Molecular & Cellular Proteomics

The SysteMHC Atlas project

Wenguang Shao, Patrick G. A. Pedrioli, Witold Wolski, Cristian Scurtescu, Emanuel Schmid, Juan Antonio Vizcaíno, Mathieu Courcelles, Heiko Schuster, Daniel J. Kowalewski, Fabio Marino, Cecilia S. Lindestam Arlehamn, Kerrie Vaughan, Bjoern Peters, Alessandro Sette, Tom H. M. Ottenhoff, Krista E. van Meijgaarden, Natalie E. Nieuwenhuizen, Stefan H. E. Kaufmann, Ralph Schlapbach, John C. Castle, Alexey I. Nesvizhskii, Morten Nielsen, Eric W. Deutsch, David Campbell, Robert L. Moritz, Roman A. Zubarev, A. Jimmy Ytterberg, Anthony W. Purcell, Miguel Marcilla, Alberto Paradela, Qi Wang, Catherine E. Costello, Nicola Ternette, Peter A. van Veelen, Cécile A. C. M. van, Albert J. R. Heck, Gustavo A. de Souza, Ludvig M. Sollid, Arie Admon, Stefan Stevanović, Hans‐Georg Rammensee, Pierre Thibault, Claude Perreault, Michal Bassani‐Sternberg, Ruedi Aebersold, Étienne Caron

Nucleic Acids Research

Toward an Optimized Workflow for Middle-Down Proteomics

Alba Cristobal, Fabio Marino, Harm Post, Henk W. P. van den Toorn, Shabaz Mohammed, Albert J. R. Heck

Analytical Chemistry

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